SIV DNA vaccine co‐administered with IL‐12 expression plasmid enhances CD8 SIV cellular immune responses in cynomolgus macaques
Identifieur interne : 000A75 ( Istex/Checkpoint ); précédent : 000A74; suivant : 000A76SIV DNA vaccine co‐administered with IL‐12 expression plasmid enhances CD8 SIV cellular immune responses in cynomolgus macaques
Auteurs : Jean D. Boyer [États-Unis] ; Tara M. Robinson [États-Unis] ; Michele A. Kutzler [États-Unis] ; R. Parkinson [États-Unis] ; Sandra A. Calarota [États-Unis] ; Maninder K. Sidhu [États-Unis] ; Karuppiah Muthumani [États-Unis] ; Mark Lewis [États-Unis] ; George Pavlakis [États-Unis] ; Barbara Felber [États-Unis] ; David Weiner [États-Unis]Source :
- Journal of Medical Primatology [ 0047-2565 ] ; 2005-10.
Abstract
Abstract: Current evidence suggests that a strong induced CD8 human immunodeficiency virus type 1 (HIV‐1)‐specific cell mediated immune response may be an important aspect of an HIV vaccine. The response rates and the magnitude of the CTL responses induced by current DNA vaccines in humans need to be improved and cellular immune responses to DNA vaccines can be enhanced in mice by co‐delivering DNA plasmids expressing immune modulators. Two reported to work well in the mouse systems are interleukin (IL)‐12 and CD40L. We sought to compare these molecular adjuvants in a primate model system. The cDNA for macaque IL‐12 and CD40L were cloned into DNA vectors. Groups of cynomolgus macaques were immunized with 2 mg of plasmid expressing SIVgag alone or in combination with either IL‐12 or CD40L. CD40L did not appear to enhance the cellular immune response to SIVgag antigen. However, more robust results were observed in animals co‐injected with the IL‐12 molecular adjuvant. The IL‐12 expanded antigen‐specific IFN‐γ positive effector cells as well as granzyme B production. The vaccine immune responses contained both a CD8 component as well a CD4 component. The adjuvanted DNA vaccines illustrate that IL‐12 enhances a CD8 vaccine immune response, however, different cellular profiles.
Url:
DOI: 10.1111/j.1600-0684.2005.00124.x
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:2D6BC5129725C3A72C70E64BC34F10E345343B1FLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">SIV DNA vaccine co‐administered with IL‐12 expression plasmid enhances CD8 SIV cellular immune responses in cynomolgus macaques</title><author><name sortKey="Boyer, Jean D" sort="Boyer, Jean D" uniqKey="Boyer J" first="Jean D." last="Boyer">Jean D. Boyer</name></author><author><name sortKey="Robinson, Tara M" sort="Robinson, Tara M" uniqKey="Robinson T" first="Tara M." last="Robinson">Tara M. Robinson</name></author><author><name sortKey="Kutzler, Michele A" sort="Kutzler, Michele A" uniqKey="Kutzler M" first="Michele A." last="Kutzler">Michele A. Kutzler</name></author><author><name sortKey="Parkinson, R" sort="Parkinson, R" uniqKey="Parkinson R" first="R." last="Parkinson">R. Parkinson</name></author><author><name sortKey="Calarota, Sandra A" sort="Calarota, Sandra A" uniqKey="Calarota S" first="Sandra A." last="Calarota">Sandra A. Calarota</name></author><author><name sortKey="Sidhu, Maninder K" sort="Sidhu, Maninder K" uniqKey="Sidhu M" first="Maninder K." last="Sidhu">Maninder K. Sidhu</name></author><author><name sortKey="Muthumani, Karuppiah" sort="Muthumani, Karuppiah" uniqKey="Muthumani K" first="Karuppiah" last="Muthumani">Karuppiah Muthumani</name></author><author><name sortKey="Lewis, Mark" sort="Lewis, Mark" uniqKey="Lewis M" first="Mark" last="Lewis">Mark Lewis</name></author><author><name sortKey="Pavlakis, George" sort="Pavlakis, George" uniqKey="Pavlakis G" first="George" last="Pavlakis">George Pavlakis</name></author><author><name sortKey="Felber, Barbara" sort="Felber, Barbara" uniqKey="Felber B" first="Barbara" last="Felber">Barbara Felber</name></author><author><name sortKey="Weiner, David" sort="Weiner, David" uniqKey="Weiner D" first="David" last="Weiner">David Weiner</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:2D6BC5129725C3A72C70E64BC34F10E345343B1F</idno><date when="2005" year="2005">2005</date><idno type="doi">10.1111/j.1600-0684.2005.00124.x</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-8X7H74N0-Q/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001320</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001320</idno><idno type="wicri:Area/Istex/Curation">001320</idno><idno type="wicri:Area/Istex/Checkpoint">000A75</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000A75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">SIV DNA vaccine co‐administered with IL‐12 expression plasmid enhances CD8 SIV cellular immune responses in cynomolgus macaques</title><author><name sortKey="Boyer, Jean D" sort="Boyer, Jean D" uniqKey="Boyer J" first="Jean D." last="Boyer">Jean D. Boyer</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Robinson, Tara M" sort="Robinson, Tara M" uniqKey="Robinson T" first="Tara M." last="Robinson">Tara M. Robinson</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Kutzler, Michele A" sort="Kutzler, Michele A" uniqKey="Kutzler M" first="Michele A." last="Kutzler">Michele A. Kutzler</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Parkinson, R" sort="Parkinson, R" uniqKey="Parkinson R" first="R." last="Parkinson">R. Parkinson</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Calarota, Sandra A" sort="Calarota, Sandra A" uniqKey="Calarota S" first="Sandra A." last="Calarota">Sandra A. Calarota</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Sidhu, Maninder K" sort="Sidhu, Maninder K" uniqKey="Sidhu M" first="Maninder K." last="Sidhu">Maninder K. Sidhu</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Vaccine Discovery, Wyeth, Pearl River</wicri:cityArea></affiliation></author><author><name sortKey="Muthumani, Karuppiah" sort="Muthumani, Karuppiah" uniqKey="Muthumani K" first="Karuppiah" last="Muthumani">Karuppiah Muthumani</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Lewis, Mark" sort="Lewis, Mark" uniqKey="Lewis M" first="Mark" last="Lewis">Mark Lewis</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Bioqual, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Pavlakis, George" sort="Pavlakis, George" uniqKey="Pavlakis G" first="George" last="Pavlakis">George Pavlakis</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Cancer Institute, Bethesda, MD</wicri:regionArea><wicri:noRegion>MD</wicri:noRegion></affiliation></author><author><name sortKey="Felber, Barbara" sort="Felber, Barbara" uniqKey="Felber B" first="Barbara" last="Felber">Barbara Felber</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Cancer Institute, Bethesda, MD</wicri:regionArea><wicri:noRegion>MD</wicri:noRegion></affiliation></author><author><name sortKey="Weiner, David" sort="Weiner, David" uniqKey="Weiner D" first="David" last="Weiner">David Weiner</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Primatology</title><title level="j" type="alt">JOURNAL OF MEDICAL PRIMATOLOGY</title><idno type="ISSN">0047-2565</idno><idno type="eISSN">1600-0684</idno><imprint><biblScope unit="vol">34</biblScope><biblScope unit="issue">5‐6</biblScope><biblScope unit="page" from="262">262</biblScope><biblScope unit="page" to="270">270</biblScope><biblScope unit="page-count">9</biblScope><publisher>Munksgaard International Publishers</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2005-10">2005-10</date></imprint><idno type="ISSN">0047-2565</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0047-2565</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Current evidence suggests that a strong induced CD8 human immunodeficiency virus type 1 (HIV‐1)‐specific cell mediated immune response may be an important aspect of an HIV vaccine. The response rates and the magnitude of the CTL responses induced by current DNA vaccines in humans need to be improved and cellular immune responses to DNA vaccines can be enhanced in mice by co‐delivering DNA plasmids expressing immune modulators. Two reported to work well in the mouse systems are interleukin (IL)‐12 and CD40L. We sought to compare these molecular adjuvants in a primate model system. The cDNA for macaque IL‐12 and CD40L were cloned into DNA vectors. Groups of cynomolgus macaques were immunized with 2 mg of plasmid expressing SIVgag alone or in combination with either IL‐12 or CD40L. CD40L did not appear to enhance the cellular immune response to SIVgag antigen. However, more robust results were observed in animals co‐injected with the IL‐12 molecular adjuvant. The IL‐12 expanded antigen‐specific IFN‐γ positive effector cells as well as granzyme B production. The vaccine immune responses contained both a CD8 component as well a CD4 component. The adjuvanted DNA vaccines illustrate that IL‐12 enhances a CD8 vaccine immune response, however, different cellular profiles.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li><li>Pennsylvanie</li><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Boyer, Jean D" sort="Boyer, Jean D" uniqKey="Boyer J" first="Jean D." last="Boyer">Jean D. Boyer</name></region><name sortKey="Calarota, Sandra A" sort="Calarota, Sandra A" uniqKey="Calarota S" first="Sandra A." last="Calarota">Sandra A. Calarota</name><name sortKey="Felber, Barbara" sort="Felber, Barbara" uniqKey="Felber B" first="Barbara" last="Felber">Barbara Felber</name><name sortKey="Kutzler, Michele A" sort="Kutzler, Michele A" uniqKey="Kutzler M" first="Michele A." last="Kutzler">Michele A. Kutzler</name><name sortKey="Lewis, Mark" sort="Lewis, Mark" uniqKey="Lewis M" first="Mark" last="Lewis">Mark Lewis</name><name sortKey="Muthumani, Karuppiah" sort="Muthumani, Karuppiah" uniqKey="Muthumani K" first="Karuppiah" last="Muthumani">Karuppiah Muthumani</name><name sortKey="Parkinson, R" sort="Parkinson, R" uniqKey="Parkinson R" first="R." last="Parkinson">R. Parkinson</name><name sortKey="Pavlakis, George" sort="Pavlakis, George" uniqKey="Pavlakis G" first="George" last="Pavlakis">George Pavlakis</name><name sortKey="Robinson, Tara M" sort="Robinson, Tara M" uniqKey="Robinson T" first="Tara M." last="Robinson">Tara M. Robinson</name><name sortKey="Sidhu, Maninder K" sort="Sidhu, Maninder K" uniqKey="Sidhu M" first="Maninder K." last="Sidhu">Maninder K. Sidhu</name><name sortKey="Weiner, David" sort="Weiner, David" uniqKey="Weiner D" first="David" last="Weiner">David Weiner</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A75 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000A75 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Checkpoint
|type= RBID
|clé= ISTEX:2D6BC5129725C3A72C70E64BC34F10E345343B1F
|texte= SIV DNA vaccine co‐administered with IL‐12 expression plasmid enhances CD8 SIV cellular immune responses in cynomolgus macaques
}}
| This area was generated with Dilib version V0.6.33. |  |